Download e-book for kindle: Animal Transgenesis and Cloning by Louis-Marie Houdebine

By Louis-Marie Houdebine

ISBN-10: 0470848278

ISBN-13: 9780470848272

ISBN-10: 0470862289

ISBN-13: 9780470862285

Animal Transgenesis and Cloning is a concise, balanced creation to this dynamic topic, protecting key matters and present ideas at the moment utilized in animal transgenesis and cloning. while offering the reader with the necessities of the topic, from the molecular foundation of gene constitution and serve as to healing gene cloning in people, social and moral implications of this significant sector of analysis also are thought of. Written in a transparent, obtainable variety, the ebook starts off with an advent to key molecular biology recommendations and specifically, considers strategies used particularly for cloning animals and producing transgenic animals. Later chapters research the varied theoretical, technical and moral concerns raised by means of cloning and transgenesis in either animals and people. Animal Transgenesis and Cloning: * Is an obtainable, concise advent to this dynamic box * Discusses organic, technical and moral matters relating to cloning and transgenesis * offers an updated account of the newest examine equipment and purposes. studies 'This is a suggestion for a e-book translation in a box that's basically of value and intensely topical. the assumption of the ebook, from gene to transgenic animal, to its makes use of and abuses is sound and logical ...... it's possible that i might recommend (using) a booklet just like the only proposed' Dr Janet Smith, college of Birmingham. 'Chapter one was once a travel de strength! Very concise and admirably transparent ...... i used to be quite inspired with the author's 2-page precis of eukaryotic gene rules, that is no suggest achievment!' Dr David de Pomerai, college of Nottingham. '...... I felt that this can be a good written, topical publication. It covers loads of floor ...... for my part the writer manages to supply a delightful volume of element and a good focussed remedy of every subject. i love the good judgment of the booklet, the truth that it pulls jointly the entire concerns into one readable and workable textual content' Dr Brendan Curran, Queen Mary, college of London.

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Example text

It is known that telomeres are sequences located at the end of each chromosome. These sequences protect DNA from degradation by exonucleases. In fact, telomeres are constantly degraded and restored. The balance becomes negative as the cells age, leading to a degradation of chromosomes and to cell death after about 50 multiplications. Gametes that are subjected to a lower number of cell multiplications are protected from telomere shortening. Gamete maturation might also include a lengthening of telomeres to ensure a long life for offspring.

Beyond the blastocyst stage, the embryonic cells become progressively specialized. This process is known as embryonic differentiation (Hadjantonakis and Papaioannou, 2001). During their formation, organs contain moderately differentiated cells, which are multipotent. Each of them can participate in the generation of a restricted number of cell types. The cells of the three major 36 TECHNIQUES FOR CLONING AND TRANSGENESIS CHAP 2 compartments of the embryo, the endoderm, the ectoderm and the mesoderm, are still moderately committed and are therefore multipotent.

This can be achieved in newborn animals but also potentially in embryos. In humans, the same methods can determine which of the embryos generated by in vitro fertilization harbour a mutated gene responsible for a severe disease. One cell is sufficient for this test since the genomic region bearing the mutation can be amplified by PCR and the mutation of the amplified fragment can be determined by restriction mapping or by sequencing. The isolated gene can be used to study its biological role in vitro and in vivo.

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Animal Transgenesis and Cloning by Louis-Marie Houdebine


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