Download PDF by Daniel L. Purich: Advances in Enzymology and Related Areas of Molecular

By Daniel L. Purich

ISBN-10: 0471349216

ISBN-13: 9780471349211

The Advances in Enzymology and comparable parts of Molecular Biology Series is without doubt one of the such a lot prestigious within the box, dedicated to the most recent traits in molecular biology and enzymology. each one quantity of the sequence comprises contributions from best gurus within the box. lower than Dr. Purich's editorship, which all started with quantity seventy two, the sequence has accelerated its assurance to incorporate thematic volumes targeting particular examine parts, in addition to non-thematic volumes including chapters with a extra basic allure.

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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Mechanism of Enzyme Action

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Its identification is of interest because of the light that might be thrown on the mechanism of carbon dioxide fixation. The compound can hardly be oxalacetic acid, for + 34 WERKMAN AND WOOD this acid is rapidly decomposed on heating in acid solution and very likely would have been converted to pyruvate during the steam distillation. It is evident from the above discussion that propionic acid is formed by a fixation reaction and probably by decarboxylation of a C,dicarboxylic acid that is formed by CCand C1 addition.

Even with evidence such as this, objection can be raised that the fixation was not conducted under natural conditions. The fixation reaction as represented in the above equation is a carboxylation of pyruvic acid and implies that the decarboxylation of oxalacetic acid is reversible. Until recently the only enzymes known to decarboxylate oxalacetic acid were carboxylase and the thermostable enzyme from muscle studied by Breusch (57). There is no certainty that carboxylase acts on the carboxyl next to the methylene carbon in oxalacetate.

Following this Wood et al. (78, 50), with heavy carbon, and independently Evans and S l o t i (63) with radioactive carbon, determined the position of the carbon fixed in isolated a-ketoglutaric acid. The isolated acid wm degraded by acid permanganate oxidation to succinic acid and carbon dioxide. All the fixed carbon waa in the carbon dioxide. The identical resulta of both investigations prove conclusively that the fixed carbon is exclusively in the carboxyl group alpha to the keto group. Krebs (M),convinced that pyruvate waa dissimilated in pigeon liver by a combination of oxalacetate synthesis through the fixation reaction and the Krebs cycle, had predicted that the fixed carbon would be found in both carboxyls of the a-ketoglutarate.

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Advances in Enzymology and Related Areas of Molecular Biology, Mechanism of Enzyme Action by Daniel L. Purich

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