By Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla
This quantity features a choice of protocols from many of the significant laboratories keen on stem mobile learn the world over. The study mentioned during this booklet covers themes akin to: keeping apart, characterizing and increasing dental pulp stem cells; manipulating the proliferative strength of cardiomyocytes via gene move; isolation of stromal stem cells from adipose tissue; noninvasive evaluation of phone fats and biology in transplanted mesenchymal stem cells; and cell-free remedy for organ fix. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and pointers on troubleshooting and warding off identified pitfalls.
Cutting-edge and thorough, Adult Stem Cells: tools and Protocols is a precious source for supporting researchers rework the research of stem cells into an industrialized approach that may provide sufferers with effective, secure, and least expensive phone treatments.
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Additional resources for Adult Stem Cells: Methods and Protocols
In both cases cells should be, at the moment of transfection, in antibiotic-free medium, since the presence of antibiotics negatively affects transfection efficiency. miRNA transfection protocol of rat and mouse neonatal cardiomyocytes differs only for the final concentration: 25 nM for rat and 50 nM for mouse cardiomyocytes. As far as siRNAs are concerned, the proper concentration should be tested experimentally on a case-by-case basis. 14. , 96 multiwell) plates are usually preferred for transfection, in order to use lower amounts of RNA and transfection reagents.
8. Remove the Giemsa stain and wash gently with dH2O until the water is clear (see Fig. 4d). 36 Ghada Abu Elghanam et al. 6 Flow Cytometry 1. 25% Trypsin–EDTA. 2. 9) and prepared for the sorting using BD LSRFortessa cell analyzer. Data obtained from the analyzer was analyzed using Flowjo software (see Fig. 5). This section describes the steps for harvesting the cells and the final transplantation into the mice via tail vein. 7 Cell Transplantation 1. Cells used for the transplantation are harvested shortly prior to the procedure.
1007/978-1-4939-6756-8_3, © Springer Science+Business Media LLC 2017 25 26 Ghada Abu Elghanam et al. believed to be multifactorial; certain environmental factors seem to provoke an existing genetic predisposition . Current treatments of SS are mostly palliative; no curative treatment exists until now. Therapeutic options include artificial saliva and tears, parasympathomimetic agents like pilocarpine (Salagen) to overstimulate the residual functioning salivary tissue. In more severe cases with serious systemic involvement, immunosuppressant drugs like methotrexate might be used .
Adult Stem Cells: Methods and Protocols by Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla